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HomeMy WebLinkAboutCOM 1013.003 2004-2006 COQUI FROG PLANS ch61Wd Nand 990? Comm. No. /01 303 Ref. ?o:. Ref. Uote AUG 22 2~U6 O O 0 111 c g a g c ;m 0 yz v 3W vc AD Dy m -0 -0 W z m~ cm 'C mm mIa ~D IS vD C}0 W a <O 0~ zm Z c m0 Cf: omi z, Dom, 0~ C) 0 -13 W -n 0 =W 00 CA;a =a 00 ~3 vm no ~RD m A9. ~ Z 30 D D m n z = 1 D p: 0 3 m y (D m T 00 y y 0 x ° D Om d DZ R -lA n~ Zi c Zz C'>T - 0 - o N d CO) QD 2 RD O H O • . . . . . . 0 CD x x r K m 5 m 71 K to -n m m o m 60 m O Df 0 0 CD CD O 3 a 0 CL V c J N C J N v n < CL 0 CL CL 3 j O O N. N < I C Z N 3 7 y S. r m 0 < N N N m CD 0 m w ' CD F D N CD N O m E, m ° l a o v o y o !p O (D w CD 0 0. o w > > o o j o o C) 'D G am 'm M J '0 -0 o m v ° o a n J G) N o 0 N v p m c - mo o O m CC a rn p c CD p 0. G c c d CD CL c fA 7 3 X Z " mmoxx CD ~tnK TD gvoCKC) pm0am oa0uT3 (n~z00- 'n 9 X J y 0 a 0 Q N D) l0 <'O D) (D N N N n 3 O N-0 (D aa N -0 N (D O O d A N 0 0 O -O N y N< d N N fJN. 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CD (D CD CD 0 O T go (D J rn J 3 T C 0 N O (n (D CO 0 7 C/1 N W fp T Z J y a N C a 0 C D7 Qo c N. 0 y 0, (A n i Z ='a c 5 v~ T a m J D) 'a 0 'a N CD 0 NB N O CD Ifrw O (°D `Z N CD -0 _fB O (D N N N F J N 0 0 0° O° m a) n 00 D> N I 0 N O flit ° O. CL N O ° V) C7 O p7 N 1 y d 7 d ' CO) Ul CL go I 'a -4 N N < O N' a (D J O N ) N C. N (D - INCIDENT ACTION PLAN COQUI FROG CONTROL AND ERADICATION `w.,. ISLAND OF HAWAII JULY 1, 2005 TO SEPTEMBER 30, 2006 VERSION 8/26/05 PRIORITY OBJECTIVES AND STRATEGIES OBJECTIVE 1. PROVIDE COMMUNITY SUPPORT TO CONTROL FROGS • PROVIDE MINI GRANTS TO COMMUNITIES - COUNTY OF HAWAII PROVIDE PUBLIC EDUCATION AND TECHNICAL ASSISTANCE TO CONTROL FROGS AND THEIR SPREAD - CTAHR • PROVIDE DIRECT CONTROL ASSISTANCE TO COMMUNITIES - USDA WS • PURCHASE ADDITIONAL SPRAY EQUIPMENT/CONTRACT WATER HAULING SERVICES FOR COMMUNITY LOAN PROGRAM - HIEDB • ADMINISTER SPRAY EQUIPMENT LOAN PROGRAM TO COMMUNITIES AND HOMEOWNERS - USDA WS OBJECTIVE 2. ERADICATE INCIPIENT POPULATIONS • UPDATE MAP OF FROG DISTRIBUTION USING HOTLINE DATA - BIISC/NWRC • CONDUCT RECONAISANCE AND FIELD CHECKS TO DOCUMENT FROG DISTRIBUTION - NWRC • ERADICATE POPULATIONS IN OUTLYING AREAS OF THE CURRENT DISTRIBUTION - USDA WS • ERADICATE POPULATIONS TO PROTECT HIGH VALUE NATURAL AREAS - BIISC OBJECTIVE 3. CONTROL THE SREAD OF FROGS ON COMMODITIES • ENSURE THAT PLANT PRODUCTS SOLD AT ALL RETAIL OUTLETS ARE SANITIZED AND FREE OF FROGS - HDOA PLANT PEST BRANCH • PREVENT INTERISLAND SHIPMENT OF FROGS THROUGH PLANT COMMODITIES THROUGH QUARANTINE AND SANITATION MEASURES - HDOA PQ • PROVIDE TECHNICAL ASSISTANCE TO NURSERIES, GREENHOUSES, FARMS TO ENSURE THAT FROGS ARE NOT SPREAD THROUGH PLANT COMMODITIES - CTAHR • OBTAIN DATA TO SUPPORT REGISTRATION OF HYDRATED LIME - NWRC/HDOA PESTICIDES BRANCH OBJECTIVE 4. DEVELOP AND IMPROVE METHODS TO CONTROL OF FROGS • IMPROVE CHEMICAL CONTROL OF FROGS IN THE FIELD and COMMODITIES- NWRC, HDOA PESTICIDES BRANCH • IMPROVE CONTROL OF FROGS ON PLANT COMMODITIES - CTAHR • DETERMINE ECOLOGICAL IMPACTS OF THE FROG INFESTATION - UH HILO • DOCUMENT AND DETERMINE EFFICACY OF ALL EFFORTS TO CONTROL FROG POPULATIONS - NWRC THE COQUI FROG WORKING GROUP CONSISTS OF: COQUI FROG COORDINATOR, COUNTY OF HAWAII WILLIAM KENOI, COUNTY OF HAWAII ARNOLD HARA, CTAHR WILLIAM MAUZ, UH HILO WILL PITT, NWRC KYLE ONUMA, HDOA TIM OHASHI (SHAYNE VERIATO AS ALTERNATE), WILDLIFE SERVICES The CFWG will develop objectives and strategies and is comprised of a coordinator and section chiefs charged with implementing the incident response plan. Coqui Frog Working Group Coordinator (Incident Commander) - County Contractor • A county contractor will serve as the chairperson of the CFWG and will be in charge managing the incident response plan • Coordinate activities of the county and cooperating agencies, section chiefs and community groups • Serve as spokesperson for CFWG • Serve as legislative liaison Operations Section Chief - Tim Ohashi, USDA APHIS WS • Operations will be divided into two divisions: Wildlife Services Division and BIISC Division • Wildlife Services Division will field crews to assist and support community efforts to control frogs • Wildlife Services Division will conduct direct control operations to eradicate incipient populations not covered by BIISC • BIISC Division will field crews to protect high value natural areas from infestations such as those near the national park and natural areas reserve systems. • Hawaii County Division will field crews to conduct control operations on County lands especially solid waste transfer stations • HIEDB will contract water hauling services for community operational support in catchment areas • County will provide water for community operations in non-catchment areas. • CTAHR will field one two man crew during the summer to respond to small frog infestations of 1-10 individuals during the summer Reconnaissance, Planning and Evaluation Section Chief - Dr. Will Pitt, USDA APHIS WS NWRC • BIISC will continue to obtain data from HOTLINE and will provide to NWRC • NWRC will update database and distribution map of coqui populations • Updated maps will be provided to Operations Section to plan control of incipient populations • NWRC will conduct reconnaissance activities to improve map and to assist in planning control of incipient populations • NWRC will evaluate Wildlife Services Division operations for efficacy and to recommend retreatments. Research and Development Section Chief- Dr. William Mautz, UH Hilo There will be three areas of research coordinated by the R&D Section Chief. chemical control, non chemical control, ecology NWRC will conduct chemical control research and development • CTAHR will conduct non chemical control research and development • UHHILO will conduct ecological studies • Ensure research information is shared among all section chiefs. Education and Outreach Section Chief - Dr. Arnold Hara, CTAHR • CTAHR will develop education and outreach materials and programs to inform public on all aspects of coqui frog control • CTAHR will develop press releases and other information to support CFWG activities Regulatory and Commodities Section Chief - Kyle Onuma, HDOA • HDOA will inspect, quarantine and regulate shipments of plant commodities to other islands to ensure products are free of frogs and other pests • HDOA will work with retail outlets to establish sanitation procedures to ensure that the public purchases products free of frogs • CTAHR will work with nurseries, greenhouses, farms to ensure that plant commodities are free of frogs. Finance Section Chief - William Kenoi, County of Hawaii • The County will disperse current funding to appropriate agencies, purchase equipment and supplies or delegate such tasks 0 Administer County Mini-Grant Program yr.. r C7~ r J r F HAWAII COUNTY COQUI FROG PLAN August, 2004 Draft TABLE OF CONTENTS Page 1. INTRODUCTION AND BACKGROUND 1 II. AUTHORITY A. Federal Government 3 B. State Government 3 C. County Government 3 III. PROBLEM A. Noise ..........................................................................................4 B. Ecosystem 4 C. Economy ....................................................................................4 IV. GOALS AND OBJECTIVES 6 V. ACTION STEPS A. Stop the Importation of Frogs into the State ..............................6 B. Stop the Spread of Frogs from Infested Areas - to the Rest of the State 6 C. Stop the Spread of the Frogs from Infested Areas to Other Parts of Hawaii Island 7 D. Eradicate and Control Existing Populations of Frogs 7 VI. RESOURCES A. Government Agencies 8 B. Private Industry ..........................................................................8 C. Community Groups and Resources 8 VII. GAPS IN ACCOMPLISHING GOALS AND OBJECTIVES A. Manpower ..................................................................................9 B. Equipment ..................................................................................9 C. Funding ......................................................................................9 D. Research and Development ....................................................10 E. Legislation ................................................................................10 VIII. CONCLUSION ...................................................................................10 HAWAII COUNTY COQUI FROG PLAN Draft I. INTRODUCTION AND BACKGROUND In 1992, the first sightings of caribbean tree frogs, Eleutherodactylus coqui, more commonly known as Coqui Frogs, were identified on the Island of Hawaii. It is believed that their accidental introduction to the Island of Hawaii occurred in the early 1990's when this alien species hitched a ride in potted nursery plants. The documented sightings and reports of the coqui frogs increased from eight (8) reported sights in 1998 to over eighty-five (85) by 2001. Currently, the coqui frog has established populations on the Islands of Hawaii, Maui, Oahu, and Kauai. However, the largest numbers of infestations, currently estimated at over two hundred (200) sites, are on the Island of Hawaii. Maui has over forty (40) sites. . The adverse and negative impacts from the coqui frogs are measured by its loud and noisy mating calls, its high population density, and its impact upon native species, the nursery industry, real property values, and the peace and quiet of residents and visitors alike. The coqui's loud calls are the mating sounds of the male frog and have been measured at levels exceeding 70 decibels. Combined with population densities that can exceed 8,000 individuals per acre, coqui infestations present a serious threat to the quality of life for our Island residents. Research on controlling the coqui frogs began in 1999 by the USDA National Wildlife Research Center (NWRC), Hawaii Field Station, in collaboration with the Hawaii State Department of Agriculture (DOA). Early efforts were focused on finding a chemical (pesticide / pharmaceutical) solution to eradicate and control coqui frog populations. Initially, success was found with caffeine and pyrethroids but the cost, effectiveness, and federal requirements proved to be hurdles in establishing these chemicals as effective eradication and control tools. In 2001, an ad hoc working group started an invasive Species Management Plan. 1 • This working group consisted of members from the State Department of Agriculture, Department of Land and Natural Resources, the University of Hawaii, the United States Department of Agriculture (Wildlife Services Division and the National Wildlife Research Center), the County of Hawaii, private industry representatives, and community associations. The goal was to establish a short-term, medium-term, and long-term strategic plan focused on eradication and control of coqui frog infestations. • The initial focus of the Coqui Frog Working Group (CFWG) was to increase communication between government agencies and private entities to ensure cooperation and minimize duplication of efforts to limit the spread of coqui frog populations. Bi-monthly meetings were held between community stakeholders beginning in 2002. Laboratory and field research, control and abatement efforts, chemical and biological control methods, as well as educational materials were developed over a two year period by the CFWG. • On April 12, 2004, Mayor Harry Kim declared that a state of emergency exists on the island of Hawaii due to the current and future adverse impacts of coqui frog infestations on the environment, the economy, and the health and safety of Island residents. 2 11. AUTHORITY A. FEDERAL GOVERNMENT USDA - The Wildlife Services program of the Animal and Plant Health Inspection Service, U.S. Department of Agriculture is the Federal Agency authorized to manage wildlife that threaten natural resources, agriculture and human health and safety (46 Stat. 1468, 7 U.S.C. 426). B. STATE GOVERNMENT • DOA - The Plant Quarantine program regulates the inter- island movement of plants infested with the coqui frog (HRS 150A-2 & HAR 4-72-3,4). The DOA officially declared the coqui frog a pest on September 27, 2001. The Plant Pest Control Branch is tasked with controlling or eradicating pests injurious to agriculture or vegetation. (HRS 141-3, HAR 4-69A). • DLNR - Species that are injurious to wildlife fall under the jurisdiction of the DLNR. They are tasked with enforcing the unlawful transportation or release of pests or harmful organisms into the wild, into areas where they are not established, or export from the State. (HRS Title 13, Chapter 124). • UH - The University of Hawaii, College of Tropical Agriculture and Human Resources (CTAHR) is federally mandated to fulfill the University's threefold land grant mission of instruction, scientific research, and outreach to address the State needs. C. COUNTY GOVERNMENT • OFFICE OF THE MAYOR - The County of Hawaii has jurisdiction to execute and implement programs to safeguard the health and well-being of its residents. The Office of the Mayor, as the executive agency of the County, has the authority to proclaim and declare a state of emergency if a threat to the County exists (FIRS, Chapter 127 and 128, Hawaii County Code, Chapter 7). 3 III. PROBLEM A. NOISE • The Department of Health established 70 decibels as the maximum permissible sound level before the sound is determined to be excessive noise (HRS 324F-1). • The mating calls of coqui frogs have been measured in excess of 70 decibels. • The sound of a single coqui frog has been measured at 95 decibels at 50 cm. • Population estimates for coqui frog infestations are believed to exceed 8,000 individuals per acre. • Multiple complaints have been received from residents that coqui frog infestations are disturbing their sleep patterns and adversely impacting their way of life. B. ECOSYSTEM • There is great concern that coqui frogs pose a threat to the stability of Hawaii's native forest ecosystems. T • The coqui frog could affect native bird populations by competing for prey in native forests. • The coqui frog consumes a wide variety of insects which could negatively impact native invertebrates, many of whom are already competing with non-native predators and parasitoids. There is no evidence that the coqui frog consumes mosquitoes or termites, thus there is no positive impact of their introduction into Hawaii's ecosystem. • The food web of Hawaiian forests could be irreversibly altered by dense populations of coqui frogs and their impact upon native species. • Coqui frogs could be a food source for future invasion of brown tree snakes and contribute to an explosion of snake populations if they are introduced to Hawaii. C. ECONOMY • Public and vendor concerns of purchasing plant material infested with frogs could have adverse impacts on the floriculture and nursery industry. • The Island of Hawaii, with over $40 million in sales, ranks number one in the State, with most of the nurseries in East Hawaii, where coqui frog populations are highest. 4 • Shipments of plants to Oahu have been returned to Hawaii Island after they were found to be infested with coqui frogs. • A nursery shipment was destroyed in Guam after inspection revealed that coqui frogs were contained in a container. • Hawaii Island Board of Realtors has expressed a desire to eradicate and control coqui frog populations because of the impact that the frog is having on sales and purchases of real property. • A recent survey of realtors revealed that between $4.6 to 6.5 million dollars of real estate transactions have been affected because of coqui frog infestations. • The impact of coqui frogs upon the visitor industry is not known at this time but there is concern that there could be an adverse impact if Hawaii becomes known for harboring noisy pests. 5 IV. GOALS AND OBJECTIVES A. STOP THE IMPORTATION OF FROGS INTO THE STATE B. STOP THE SPREAD OF FROGS FROM INFESTED AREAS TO THE REST OF THE STATE C. STOP THE SPREAD OF THE FROGS FROM INFESTED AREAS TO OTHER PARTS OF HAWAII ISLAND D. ERADICATE AND-CONTROL EXISTING POPULATIONS OF FROGS V. ACTION STEPS A. STOP THE IMPORTATION OF FROGS INTO THE STATE • More stringent inspection of incoming cargo from mainland and foreign origins. • More USDA and DOA personnel to execute inspections of airport and harbor shipments. • Stricter fines and penalties for importation of frogs by importers and exporters of materials that harbor frogs. • Dissemination of information and education of businesses to avoid unintentional shipment of coqui frogs. • Federal and State legislation to implement action steps. B. STOP THE SPREAD OF FROGS FROM INFESTED AREAS TO THE REST OF THE STATE • More DOA personnel to conduct random inspections of cargo containing nursery or plant material. • More DLNR personnel to conduct enforcement of violations of harboring and/or transporting declared pests. • Stricter laws to put teeth into existing legislation regarding the harboring and transportation of coqui frogs. • Self-policing and education of wholesale and retail outlets of potted plants and nursery materials to avoid the spread of frogs to uninfested areas. 6 C. STOP THE SPREAD OF THE FROGS FROM INFESTED AREAS TO OTHER PARTS OF HAWAII ISLAND Spray and clean up transfer stations, which are infested with coqui frogs, and serve as a source of unintentional transport for coqui frogs Work closely with nurseries, retail outlets, and transportation companies to avoid transport of frogs to other parts of Hawaii Island Public education campaign utilizing all forms of media to inform public about the different ways that coqui frogs can be unintentionally transported Identify all infestations on Island to contain existing sites and avoid growth and spread of coqui frogs D. ERADICATE AND CONTROL EXISTING POPULATIONS OF FROGS Support and encourage the USDA Wildlife Services to implement their eradication and control plan that is focused on coqui frogs on Hawaii Island. Work with the Hawaii Invasive Species Council to encourage the DOA and DLNR to conduct eradication and control campaigns on State lands and in State parks. Create County Task Force made up of county personnel to conduct eradication frogs on County land (parks, dumps). Empower and support community eradication and control efforts through loan sprayer program. 7 VI. RESOURCES A. GOVERNMENT AGENCIES • Federal - Funding request has been approved by the USDA and is awaiting final approval from the Office of Management and Budget in the amount of $3,000,000 a year for 3 years. Monies are allocated for coqui frog control for Hawaii Island. • State - The Legislature passed a funding bill for the HISC this past legislative session for $3,000,000 that is intended to cover all invasive species for all Islands. • County - The County appropriated $45,000 for an island- wide loan sprayer program that is intended to support community based efforts to eradicate and control coqui frog populations. B. PRIVATE INDUSTRY • Currently, individual nurseries have been using their own resources to clean, protect, and control coqui frogs on their properties and within their businesses. • Realtors have been meeting and are looking for ways to support coqui frog control efforts. • The Hawaii Visitors and Convention Bureau has been contacted and asked to determine level of support of the visitor industry to participate in control efforts. • A couple of private parties have been attempting to provide a fee for service for residents and businesses interested in hiring contractors to perform coqui eradication and control. To date, no significant effort has been established. C. COMMUNITY GROUPS AND RESOURCES • Hawaiian Paradise Park residents have established a Frog Squad to control coqui frogs in their subdivision. To date, they have raised nearly $30,000 from a small percentage of property owners in the subdivision. They are working closely with the USDA and the CFWG. • Kohala residents have raised over $15,000 for coqui frog eradication in their area. They have been successful thus far in eradicating and controlling coqui frogs in Kohala. • Kapoho residents have been using their own resources to develop eradication and control techniques. They have also been successful with their efforts thus far 8 VII. GAPS IN ACCOMPLISHING GOALS AND OBJECTIVES A. MANPOWER • To date, there is a shortage of manpower at every level. Federal agencies, State agencies, and County agencies all complain of a lack of dedicated manpower for coqui frog control efforts. The Big Island Invasive Species Council (BIISC) has recently lost all of its personnel because of budget cuts. Community manpower is limited to specific, motivated areas. Other communities, with elderly populations, want to help control coqui frogs but are physically unable to do the work. • Each agency needs to commit a minimum amount of manpower to this effort - at least to address coqui frog populations on lands under their jurisdiction. • Residents continue to call various departments looking for governmental action but are disappointed when there is no "strike force" to respond to their requests. Ideally, private businesses will find it worth their effort to focus on coqui frog control and eradication. B. EQUIPMENT • Power sprayers for coqui control have been purchased by the DOA (4), community group Malama O Puna (2), and soon, by the County and USDA (8). This will allow for community groups and residents to use this equipment for coqui control efforts in their neighborhoods • Citric Acid and Hydrated Lime are effective chemicals that can be used for coqui frog infestations. However, citric acid can be costly and hydrated lime, although legal and widely available, has not been certified as a registered pesticide by the EPA and thus cannot be used by government agencies. • Power drenchers are being experimented with to determine if they are effective in controlling coqui. The DOA and UH will know its effectiveness by the end of the year. C. FUNDING • The legislative reference bureau, when tasked by the legislature to report on invasive species, reported that federal, state, and private agencies greatest challenge in addressing invasive species is the same - "money". 9 The Coordinating Group on Alien Pest Species, in the same legislative report, stated that funding for invasive species is an "ongoing" cost and that to effectively fight invasive species in Hawaii for one year would require $49,847,000. D. RESEARCH AND DEVELOPMENT • Dr. Larry Woolbright, Professor from Sienna College in New York and pre-eminent scholar on the coqui frog, will be taking a research sabbatical from his job and spending a year, 2005, in Hawaii doing research on the impact of the coqui frog in Hawaii. • Dr. Eloise Killgore, DOA, is continuing research into chytridiomychosis, a fungus that kills frogs, and its viability as a biological control of the coqui frog in Hawaii. • Dr. Arnold Hara and Dr. Bill Maus (UHH) are continuing their research into the biology of the coqui frog and methods of destroying coqui egg clutches before they hatch. Also, they are continuing to determine the efficacy of habitat modification. • Dr. Bob Sugihara and Dr. Will Pitt are continuing their research to discover other chemical/pharmaceutical methods of eradication and control of coqui infestations. E. LEGISLATION • The Hawaii Invasive Species Council, co-chaired by the Directors of the DOA and DLNR, and including representatives from each county and all relevant departments (DBEDT, DOD, DOT, DOH) are tasked with identifying and drafting relevant legislation for invasive species impacting the State of Hawaii. VIII. CONCLUSION • It is critical that resources and manpower be dedicated towards coqui frog control by all levels of government. It is also important to include the private sector and community members in addressing this problem. 10 I J'Z1 Or - V' 4 Harry Kim Dixie Kaetsu Mayor Managing Director ~W+R" •r a. ` ir• eiN~~ Peer L. Hendricks Deputy Managing Director County of Hawaii 25 Aupuni Street, Room 215 • Hilo, Hawaii 967204252 • (808) 961-8211 Fax (808) 961-6553 KONA: M6 Kuakini High::ay, Suite 103 Kai!ua .Kona, Hawa:'i 96740 (808)329-5226 • Fax (808)326-5663 WHEREAS, the Department of Health establishes 70 dBA as the maximum permissible sound level before the sound is determined to be excessive noise as defined in Hawai'i Revised Statutes Section 342F-1; and WHEREAS, the Island of Hawaii has become infested by an invasive species of Caribbean tree frog, Eleutherodactylus coqui, more commonly known as Coqui frogs, since their accidental introduction on the Island of Hawai'i in the early 1990's; and WHEREAS, in early 1992 there were only sporadic sightings of coqui frogs, in 1998 there were eight reported occurrences, in 2001 over 85 occurrences were documented with another 65 occurrences that were undocumented, and now there are over 150 firmly established specific sites on the Island of Hawaii; and WHEREAS, in their native range on Puerto Rico, coqui populations can reach densities greater than 8,000 individuals per acre and consume an estimated 47,500 prey per night per acre, and because Hawai'i is similar in climate to Puerto Rico but without any natural predators, it is estimated that populations on the Island of Hawai'i can reach ten times that reported for coqui in the native forests of Puerto Rico; and WHEREAS, the infestation of Coqui frogs is in such concentrations that the sounds they emit have been measured at sustained levels exceeding the Department of Health's maximum permissible sound level of 70 dBA, and therefore have become a threat to human health and welfare and unreasonably interferes with the comfortable enjoyment of life and property; and WHEREAS, it is unknown to what extent this invasive species will impact on the native and endemic species of plant and animal and local ecosystems throughout the Island of Hawai'i but researchers with the University of Hawaii have stated that with the amount of food that a coqui population consumes per acre per night, that it will irreparably harm our fragile ecosystem; and WHEREAS, the Island of Hawai'i is known for its unique endemic ecosystems with flora and fauna that are found nowhere else in the world and it is unknown what effect this invasive species will have on these ecosystems; and WHEREAS, the excessive noise emitted from Coqui frogs has caused a threat ! to the economic welfare of the Island of Hawai'i due to the possibility of quarantine restriction and de-infestation measures being placed on floral and plant exports, upon the important visitor industry, and upon sales of property; and WHEREAS, the Coqui was declared an agricultural pest on September 27, 2001, making shipments of plants infested with Coqui subject to quarantine according to Chapter 150A-2 of the Hawai'i Revised Statutes and Sections 4-72-3 and 4 of the Hawaii Administrative Rules, which has the potential to negatively impact grower sales of HawaiTs floriculture industry which totaled $61.187 million in 2002, the majority of growers being located in East Hawai'i where the Coqui frog infestation is greatest; and WHEREAS, the brown tree snake, which has devastated the ecosystem of Guam, but has not yet established populations in the State of Hawai'i, will have a readily available and abundant supply of food because of Coqui infestations and will allow the brown tree snake to not only establish a foothold, but allow any infestation to grow because of the density of populations of Coqui in certain parts of the Island of Hawai'i; and WHEREAS, the State of Hawai'i Department of Agriculture, the State of Hawai'i Department of Land & Natural Resources, the University of Hawai'i College of Tropical Agriculture and Human Resources, and the United States Department of Agriculture have been working closely with private sector businesses and communities to try to address the Coqui frog infestation with very limited financial resources, and have expressed the need for an influx of resources to mitigate this problem; and WHEREAS, the State of Hawaii Legislature enacted Act 85 in 2003 establishing the Hawai'i State Invasive Species Council (HISC), providing statutory authority to the Council to continue its special purpose to foster and organize coordinated approaches among various executive departments, federal agencies, and international and local initiatives for the prevention and control of invasive species and to affirm the objective of the State to rid Hawai'i of invasive species; and WHEREAS, the 2003 State Legislature authorized the creation of the Council and stated that the invasion of Hawaii by alien species is the single greatest threat to HawaiTs economy, natural environment, and the health and lifestyle of HawaiTs people and visitors; and WHEREAS, Hawai'i is one of only four states in the entire country that has recognized the need for coordination among all state agencies, at a cabinet level, that have responsibility to control invasive species on the ground, as well as regulate or promote the pathways in which invasive species can gain access into the State of Hawai'i; and WHEREAS, due to the threat that excessive noise emitted by the Coqui frogs poses to human health and welfare, the unknown impact of the Coqui frogs on the Island of Hawai'i's ecosystems, as well as the threat to the economic welfare of the Island of Hawai'i, and the need for government agencies and representatives from the private sector to mobilize and provide immediate services to our island residents, a civil defense state of emergency is authorized pursuant to Chapters 127 and 128, Hawai'i Revised Statutes, as amended, and Chapter 7, Hawaii County Code. NOW, THEREFORE, I, HARRY KIM, Mayor of the County of Hawaii, do hereby proclaim and declare that a state of emergency exists on the Island of Hawai'i, effective I=oo p.m., April v~- 2004, and continuing thereon until further act by this office. IN WITNESS WHEREOF, I have hereunto set my hand and caused the Seal of the County of Hawai'i to be affixed. Done this I2'- day of ARri 1 2004, in Hilo, Hawai'i. HARRA YKI'b}- Mayor County of Hawai'i COUNTY OF HAWAII Emergency Action Plan for Coqui Frog Short Term Plan Goals & Objectives: Immediately assist ongoing efforts by Big Island communities to mitigate coqui frogs in their own neighborhoods. Accomplishments: 1) Reduce the noise around communities, 2) reduce the build-up and spread of frogs, 3) show that the County is supportive of community programs, and d) gain support for overall frog control project. County of Hawaii: 1) designated a Coqui Frog Control Coordinator, 2) purchase 8 truck- mounted sprayers @ $3,000 each for $24,000 total: 4 sprayers to be loaned out to community groups for control on private lands, and 4 for County personnel to control frogs on County lands, 3) purchase citric acid (hydrated lime when applicable) for $26,000 total for use by County and community groups on a subsidized or cost-share basis. Hawaii Department of Agriculture: 1) Assist in providing statewide coordination during interim until Hawaii Invasive Species Council provides oversight, 2) continue efforts to develop an exemption for use of hydrated lime to control frogs, 3) assist in educating the public and County personnel on controlling coqui frog, 4) continue Sprayer Loan Program to assist communities, 5) assist nurseries and retail outlets to stop the spread of frogs on and between islands, and 6) continue applied research on innovative strategies to control frogs. UH-CTAHR Manoa / Coqui Frog Working Group: 1) Continue research on frog biology, habitat modification, and other control methods, 2) continue outreach and education of nurseries, communities and general public, 3) solicit and gain political/public support of project, and 5) advise County Coqui Frog Control Coordinator. Private Parties: 1) Nurseries and other agricultural producers to control frogs on their property and on plants prepared for distribution; 2) plant retailers to sell frog-free plants; and 3) agricultural retail sales to provide reduced price for bulk shipments of chemicals, use existing facilities to assist with storage and delivery, and help develop a plan for the orderly distribution of chemicals to communities. Communities in Greatest Need: Kurtistown, Mt. View, Pahoa, Ainaloa, Orchidland, Hawaiian Paradise Park, Papaikou, Honaunau, Kainaliu, and Kukuihaele. Total Cost: $ 24,000 - 8 100-gal. sprayers or 2-3 drenchers (hydrated lime only) 26,000 - for citric acid or hydrated lime (when applicable) $ 50,000 -Total Cost 1 Medium Term Plan Goals & Obiectives: With the Hawaii Invasive Species Council providing oversight, develop a statewide coqui frog control plan with community groups and other County, State and Federal agencies to address this problem on all islands. Accomplishments: 1) Target specific County, State and Federal lands for frog control to motivate all agencies and individuals to cooperate in the ongoing frog control program, and 2) get other counties to follow suit by providing leadership by example. County of Hawaii: 1) Coordinate and mobilize major frog control efforts where applicable in various hot spots on the island, similar to the Lake Wilson effort, with personnel from the County, State, Federal, Big Island Invasive Species Committee, private parties, and military, 2) consider use of other strategies including aerial spraying, controlled burning or land clearing with heavy equipment (dozers, cranes, dumpsters), and 3) provide the equipment and logistical support to carry out these projects. Department of Agriculture: 1) Support the Hawaii Invasive Species Council which provides statewide oversight, 2) continue applied research to control the frog, 3) continue to assist nurseries and retail outlets to stop the spread of frogs on and between islands, 4) continue to inspect and monitor plants moving between islands for frogs, 5) develop a monitoring program for intra-island shipments of plants at designated sites on island, and 6) continue work on biocontrol. UH-CTAHR Manoa / Coqui Frog Working Group: 1) Continue research and educational outreach programs on frog, and 2) provide media with updates on project. Department of Land and Natural Resources: 1) Support the Hawaii Invasive Species Council which provides statewide oversight, 2) clear and treat frog-infested state lands under their jurisdiction, especially those lands adjacent to communities, and 3) enforce injurious wildlife laws pertaining to the intentional moving or harboring of the coqui frog. Department of Health: 1) Support the Hawaii Invasive Species Council which provides statewide oversight, 2) grant open burning permits where applicable to reduce frog habitats, and 3) treat frog-infested lands under their jurisdiction, especially those lands adjacent to communities. USDA - Wildlife Services: 1) Educate regarding need for more federal monies to control the frog, 2) work with county to develop Environmental Assessment or Environmental Impact Statement to conduct large scale projects listed above, 3) continue to obtain and provide testimony on the seriousness of this invasive species, 4) continue research on alternative toxicants for the frog, and 5) continue to maintain hotline with BIISC on frogs. Big Island Invasive Species Committee: 1) Maintain frog hotline, 2) continue database and mapping of frog populations, 3) assist USDA-WS and Volcanoes National Park 2 r' personnel in treating that and surrounding areas, and 4) assist in treating remote areas where rappelling may be necessary. Areas in Greatest Need: County, State and Federal lands that are adjacent to communities and/or other areas where active frog control is being practiced. Total Cost: $500,000 - funds to be disbursed by Coqui Frog Control Coordinator to County, State and Federal agencies assisting the county in controlling frogs on County, State and Federal lands on the Big Island. Long Term Plan OPTION A Goals & Objectives: Control the coqui frog biologically by releasing a pathogen or other natural enemy that targets the coqui frog. Accomplishments: 1) Obtain chytrid fungus or other natural enemies of the coqui frog for potential biocontrol, 2) test natural enemies of the coqui frog in a Quarantine Facility for effectiveness and possible impact on non-target species, 3) request and receive approval for release from quarantine, 4) release and evaluate effectiveness of biocontrol agent. County of Hawaii: Continue to support ongoing efforts to biologically control the coqui frog. Department of Agriculture: 1) Obtain chytrid fungus and/or search for other potential biocontrol agents, 2) test suitability in quarantine, 3) request and receive approval for release, 4) release and evaluate effectiveness of biocontrol agent. UH-CTAHR / Coqui Frog Working Group: Support department's biocontrol efforts. Areas in Greatest Need: Entire State of Hawaii. Total Cost: $ 10,000 - cost to retrieve chytrid fungus 35,000 - explorations in Puerto Rico for natural enemies of frog 5,000 - incidentals $ 50,000 -Total Cost OPTION B Goals & Objectives: Control the coqui frog genetically by 1) incorporating new and potentially deleterious genes into the genetic makeup of the coqui frog population, 2) 3 "knocking out" or silencing developmental genes arresting frog development leading to death, or 3) develop reproductive sterility in coqui frogs. Accomplishments: 1) By manipulating coqui-specific DNA sequences, design a short sequence that will prove lethal to developing embryos, 2) formulate RNAi (interference) that can be applied in the field as a species specific growth inhibitor of coqui frogs, and 3) develop a chemosterilant that could be specific to the coqui when applied in the field. County of Hawaii: Continue to support ongoing efforts to genetically control the coqui frog. UH-CTAHR / Coqui Frog Working Group: 1) Acquire graduate students and post doctorate researchers and technicians to conduct research, 2) contract research with a leading frog genetic research laboratory, 3) release and evaluate effectiveness of genetic control. Department of Agriculture: Support the college's genetic control efforts. Areas in Greatest Need: Entire State of Hawaii. Total Cost: $ 300,000 - personnel 50,000 - equipment 100.000 - materials & supplies $ 450,000 - Total Cost (3-year budget) 4 Coqui Frog Control Plan for State of Hawaii February 2004 by Hawaii Department of Agriculture Coqui Frog Working Group Purpose - To control the coqui frog on all islands in the State of Hawaii where it is causing nuisance, environmental and economic problems by its sheer numbers and loud mating calls. Strate -The coqui frog problem is larger than what can be handled by any single government agency. This strategy relies on the public to help control the frog on private lands and government agencies to control frogs on public lands. Since the frog is not equally distributed throughout the State and each county may have specific priorities for control, the coordination of control activities in each county is best determined by the county itself with technical assistance from Coqui Frog Working Group (CFWG) that was organized in Hilo, Hawaii in June 2002 to address the coqui frog crisis. The purpose of the CFWG is to serve as a source of diverse technical and scientific expertise, provide a forum for sharing results of research, encourage networking between various government agencies (i.e. federal, state, county) and the private sector (i.e. nurseries, retail outlets etc.), consolidate government agencies' manpower, assets, and expertise to improve cost effectiveness, and to reduce redundancy of multi organizational efforts in controlling the coqui frog. Three Point Plan - Funding can be designated to one entity that disburses funds to each county or be disbursed directly to each county. Funding should be based on need, be used to address the immediate problems first, and used in a way that results in the greatest impact with the. limited funds available. 1. Empower the public to control the frog on their own properties (short- term goal). A. Each county needs to provide the necessary equipment and supplies to control the frog, and designate a Coqui Frog Coordinator to disseminate frog control information and coordinate control activities in that county. - Coqui Frog Working Group will include representatives from each county and advise the county coqui frog coordinators. - Univerisity of Hawaii (UH), Hawaii Department of Agriculture (HDOA) and the Invasive Species Committees in each county (ISC) will assist counties during interim by providing knowledge about the frog and its weaknesses, and control strategies. B. Each county needs to develop a Sprayer Loan Program for their islands. 1 - Counties needs to purchase large power sprayers to loan out to community associations and other volunteer groups. Decision needs to be made by each county as to whether county can purchase citric acid or other toxicants, or whether community groups need to purchase their own. C. Community associations and volunteer groups need to take responsibility for controlling the frog in their own backyards where frogs are causing problems. - Groups need to get permission from property owners before spraying, forewarn property owners that spraying is to take place, and spray in a responsible manner. - Groups need to borrow equipment from county and be prepared to purchase citric acid or other toxicant on their own accord. D. Resources needed for each county (Update needed). $50,000 for Hawaii (8 power sprayers @ $3,000 each + toxicants) - $15,000 for Maui (2 power sprayers @ $3,000 each + toxicants) $15,000 for Kauai (2 power sprayers @ $3,000 each + toxicants) - $15,00 0 for Oahu (2 power sprayers @ $3,000 each + toxicants) $95,000 Total for all counties 2. Empower government agencies to control the frog on public lands for which agencies are responsible (medium-term goal). A. Leadership is needed for all county, state, and federal agencies within a county to get involved to do their part in controlling the frog on their lands which they manage or are responsible. - Target specific County, State or Federal lands for cooperative control effort. - Coordinate and mobilize major frog control effort to provide leadership and to motivate participants. - County Coordinators and their Mayor's Offices need to meet with and influence other agencies to work together in reaching a common goal to reduce frog numbers in problem areas. B. Government agencies need to know how the frog directly impacts their missions and responsibilities, i.e., the frog's impact on the nursery industry, or the frog's impact on native insects that serve as food for native 2 birds and pollinators of native plants; or the frog's impact on the social well-being and health of a public that is exposed to the constant noise; or the frog's impact on land values, property tax and tourism due to its noise. - UH, HDOA and others need to assist in developing the "story C. County, state, and federal agencies need to start spraying on their lands, especially those lands that border communities that are trying to control the frogs themselves. - Affected agencies need to designate personnel and set aside funds for sprayers and citric acid, or if need be, redirect existing funds to control frogs. D. Reduce the movement of frogs from one island to another or from one location to another. - HDOA needs to strengthen interisland quarantine inspections. - Other agencies need to enforce existing rules on the movement or transport of pests or injurious animals from one location to another. E. Resources needed for all counties (Update needed) - $500,000 for County of Hawaii - 100,000 for Maui, Kauai and Oahu $600,000 Total for all counties 3. Continue the research on finding a more cost-efficient control solution for the frog (long-term goal). A. Seek Quarantine Exemption for use of hydrated lime as a frog toxicant. (Accomplished). - HDOA is running tests with hydrated lime and citric acid and will push for exemption B. Screen registered pesticides for use against coqui frogs. (Ongoing) - USDA National Wildlife Research Center and HDOA are conducting trials. C. Search and study internal parasistes of coqui frogs from Puerto Rico in a long-term biocontrol program (Ongoing) 3 UH and HDOA to conduct quarantine studies on potential biological control agents. D. Research ways to treat plants for frogs before export, movement, or sale. - UH-CTAHR is conducting research on hot water treatments and other methods (Accomplished). E. Investigate barriers and traps to prevent frogs from infesting nurseries. (Ongoing) F. Research ways to control the frog genetically. - UH, other universities or research centers with expertise in frog genetics should conduct research to control coqui frog by incorporating new deleterious genes in frog population, by "knocking out" developmental genes, or by inducing reproductive sterility in the coqui frog (sterility by irradiation is planned by UH- CTAHR) G. Resources needed to conduct research (Update needed). $ 50,000 for UH and HDOA biological control of frogs 450.000 for genetic control of frogs $500,000 total costs Total Resource Needs (Update needed) Resource needs for short- and medium-term control are for first-year only. Subsequent year costs are dependent on quality of treatments and the weather. Long-term control costs are for the first 3 years. $ 95,000 Short-term Control 600,000 Medium-term Control 500,000 Long-term Control $1,195,000 Total Cost of Statewide Frog Control Program. 4 t)epartment USDA ofAgriculture. STATE OF HAWAII Invasive Carribean Tree-Frog (Eleutherodactylus) Eradication and Control - State of Hawaii Invasive Species Management Plan Draft Prepared by: United States Department of Agriculture Animal and Plant Health Inspection Service Wildlife Services State of Hawaii Department of Agriculture Ver. March 31, 2003 or.r Executive Summary This Invasive Species Management Plan was developed to control Caribbean tree frogs from commercial nurseries and eradicate incipient populations found in the state of Hawaii. Hawaii's climate is similar to the frogs native range of Puerto Rico. The frog populations are expected to reach 8,000 individuals per acre and consume an estimated 47,500 prey per night which will pose a threat to native forest ecosystems. High densities of tree frogs can reach a noise level of 70 decibel's which may affect residential land values and the tourism industry in Hawaii. Public concern over purchasing plant material infested with the tree frogs is beginning to have an adverse impact on the floriculture and nursery product industry. Tree frogs are found on the island of Hawaii, Oahu, Maui and Kauai. Established sites include commercial plant nurseries, residential areas, resorts and hotels, public parks and within forest areas. The frogs are being spread primarily through transportation of infested plant material. Research on controlling the tree frogs began in 1999 by the USDA National Wildlife Research Center (NWRC), Hawaii Field Station. Laboratory tests were conducted to evaluate substances that might be used in controlling the frogs. NWRC found that dermal exposure to a 2% solution of caffeine and water caused 90% or greater mortality. As a result of the caffeine tests, a one year Federal Insecticide Fungicide and Rodenticide Act (FIFRA), Section 18 Specific Emergency Exemption was issued by the U.S. Environmental Protection Agency (EPA) on September 27, 2002. Hawaii Department of Agriculture Pesticides Branch imposed additional restrictions to caffeine use. Because of the restrictions there was no operational use of the caffeine to control the frogs for most of the exemption. The authorization expired on September 27, 2002. Wildlife Services (WS) has applied for a FIFRA Section 18 Quarantine Exemption to continue to make caffeine available, to allow nursery growers to use caffeine in solution to control frogs on plant products. Further research by NWRC found that citric acid at a concentration of 16% was also effective at killing Caribbean tree frogs. Citric Acid is an exempt active ingredient according to the EPA. Currently, citric acid has been cleared by HDOA for general use to control Caribbean tree frogs. Due to very high populations of coqui frogs at Lava Tree State Park a multi-agency pilot project was implemented on December 2002. Labor crews cleared all non native understory and subcanopy vegetation and research crews sprayed a 16% solution of citric acid later at night. The project showed that vegetation removal along with the citric acid spraying was highly effective against frogs in a control operation. The use of citric acid will prove to be a valuable tool to the residential property owner. The application of hot water at 45 degrees Celsius to plants for 3 minutes was also found lethal. Further tests showed that Vapor Heat Treatments were also very effective. Hawaii Board of Agriculture has declared the coqui frog an agricultural pest, thereby restricting the movement of plant products between the islands if they are infested with the frogs. Recently two shipments of plants arriving on Oahu were found to be infested with frogs were sealed and returned to the island of Hawaii at the shipper's expense. The transport of frogs around the state is an illegal activity, but the law requires proof of intent to deliberately spread the frog, which is difficult to prove. Despite this warning, frogs are still being dispersed through the potted and cut plant trade, through wholesalers and retail garden outlets. Currently Wildlife Services, CTAHR and the Hawaii Department of Agriculture are actively working with community and neighborhood associations to provide technical assistance in controlling frog infestations on the island of Hawaii. When funded this ISMP proposes to apply control and eradication efforts over the next 4 years, on the coqui and green house frogs to reduce and eliminate their numbers in an integrated pest management approach. Carribean Tree Frog Control and Eradication 2 I Introduction This Invasive Species Management Plan (ISMP) was developed to eradicate and control populations of Caribbean tree frogs (Eleutherodactylus coqui and E. planirostris) in the State of Hawaii. It incorporates a previous plan for controlling the frogs that was produced by an ad hoc working group consisting of the Hawaii Department of Agriculture (HDOA), Hawaii Pest Control Association, Wildlife Services National Wildlife Research Center, hotel industry representatives and the County of Hawaii (Hawaii Export Nursery Association 2001). The current ISMP was further developed after discussions and reviews by the County of Hawaii, the University of Hawaii Cooperative Extension Service, Hawaii Department of Agriculture, the National Wildlife Research Center and industry representatives. Plan revisions were made after the implementation of a successful multi-agency pilot project at Lava Tree State Park in Pahoa, East Hawaii to control the Caribbean tree frogs using vegetation removal and citric acid. The objectives of the proposed Plan are to reduce or eliminate the high density populations near commercial nurseries and eradicate incipient populations wherever they occur. 11 Need for Action In its native range on Puerto Rico, coqui populations can reach densities greater than 8,000 individuals per acre (20,000 per hectare) and consume an estimated 47,500 prey per night per acre (114,000 prey per night per hectare) (Kraus et al. 1999). Since Hawaii's climate is similar to that of Puerto Rico, it is expected that the frogs will also reach these or higher densities in Hawaii (HDOA 2001 a). At one collection site on the island of Hawaii, 105 frogs were collected from a 538 ft2 (50 m2) area in 50 minutes (Kraus et al. 1999). Based on this collection rate, the population estimate for this site is believed to be ten times that reported for coqui in native rainforests in Puerto Rico (Kraus et al 1999) There is great concern that Caribbean tree frogs pose a threat to the stability of Hawaii's native forest ecosystems. The majority of native birds are partially or wholly insectivorous. The Caribbean tree frogs could indirectly effect some populations of these birds if the frogs spread to native forest bird ranges. None of the known sites are inhabited by endangered species but there is potential for the frogs to enter areas where threatened or endangered species are present (HDOA 2001a). Coqui have been found in Volcano Village, which is adjacent to Hawaii Volcanoes National Park and nearby Kilauea Forest Reserve where endangered species are present. High densities of the coqui frog in residential areas have become a noise nuisance due to the male's loud calls which can reach 70 decibels. Complaints have been reported from residents and tourists who are disturbed by calling frogs (Kraus et.al. 1999). There is concern that the coqui frogs will impact the tourism industry and have a negative effect on property values. Vendor and public concern over purchasing plant material infested with frogs is beginning to have some impact on the floriculture and nursery products sales. Grower sales of Hawaii's floriculture and nursery products totaled a record $75.4 million in 1999. Hawaii is now ranked third in the nation in volume of sales, exceeded only by Florida and California. Hawaii Carribean Tree Frog Control and Eradication 3 Island, with $41.6 million in sales, ranked number one among the islands in 1999 with most M of the nurseries located in East Hawaii which is the core frog infested area (HASS 2001). III Actions Taken The coqui and greenhouse frogs are two relatively recent invasive species introduction$ to Hawaii (Kraus, et.al. 1999). They are both native to the Caribbean, although one or both have become established in the states of Florida, Louisiana and Alabama (Kraus et.al. 1999). Unconfirmed reports of one or more of these species were first reported in Hawaii as early as 1988 (Kraus et. al. 1999). In early 1992 there were only sporadic sightings of the Caribbean tree frogs on the island of Hawaii with later reports of some nurseries on Maui. In early 1998, eight occurrences were reported, but 2.5 years later, over 85 occurrences were documented and another 65 were reported but unconfirmed (HDOA 2001b). The coqui is now firmly established at more than 200 specific sites on the island of Hawaii and about 40 sites on Maui. Incipient populations are also reported on Kauai and Oahu. The greenhouse frog more widespread than the coqui frog and is found on all the major Hawaiian Islands. The established sites include commercial plant nurseries, residential areas, resorts and hotels, public parks and sites within forests. The Caribbean tree frogs are being spread to additional sites primarily through the transportation of infested plant material (Kraus et.al. 1999). There are also unconfirmed reports of deliberate dispersal of tree frogs by individuals (HDOA 2001b). Research Research on controlling the tree frogs began in 1999 by the USDA National Wildlife Research Center (NWRC), Hawaii Field Station, under contract with HDOA. Laboratory tests were conducted to evaluate more than 40 commercially registered pesticides and selected household and pharmaceutical substances that might be used in controlling tree frogs in Hawaii (Campbell 2001 a). Caffeine and resmethrin (a synthetic pyrethroid based aerosol pesticide) showed success in killing the tree frogs in the laboratory. Despite its effectiveness, the Resmethrin aerosol product was not considered a likely candidate for the control of tree frogs in Hawaii. Designed for spot treatment of plants in the nursery or around the garden, the aerosol product would be costly and impractical for wide- scale control of tree frogs (Campbell, 2001 a). Dermal exposure to 1-2 percent caffeine to water caused 90% or greater mortality to both species of frogs. Field efficacy trials subsequently were conducted by NWRC to test the application of caffeine solutions for controlling the frogs in floriculture and nursery crops in Hawaii (Campbell 2001 b). These trials showed that a single spray application of 2 percent concentration caffeine solution caused a 90-100% decline in the relative abundance of tree frogs (Campbell 2001 b). Concentrations down to 0.5 percent were tested with similar success depending on the dermal exposure, density and type of foliage (HDOA 2001b). Carribean Tree Frog Control and Eradication 4 Based on these results, NWRC and HDOA requested and received a Section 18 exemption to use caffeine. However, due to its restricted use labeling requirements operational use was never adopted in Hawaii. Citric acid has shown some promise in proving lethal to Caribbean tree frogs under laboratory conditions. Citric acid is a pesticide substance that is exempt from FIFRA requirements (40 CFR Part 152). NWRC initially tested citric acid in the laboratory after learning of its use to control Cuban Tree Frogs by a former Honolulu Zoo curator of reptiles and amphibians. Under laboratory conditions, NWRC found that it was effective at killing the two species of Carribean tree frogs at all citric acid: water concentrations at or above 16% (64, 32, 16,12, 10, 8, & 4% tested). The citric acid effect was found to be dosage dependent with differential efficacy at dosage levels less than 0.9 ml solution directly applied to the frog. Further tests were conducted by HDOA, NWRC and CTAHR to determine plant phytotoxicity, and a demonstration project at Lava Tree State Park was planned to determine its effectiveness under field conditions and to determine whether there were any adverse effects on nontarget species. Citric acid is an exempt active ingredient per 40 CFR 152.25(g), and according to EPA, no consideration needs be given to its effect on non-target species and or ground water. Water, the other ingredient in the proposed formulation is exempt per 4A of the regulation. The proposed use is exempt from federal EPA oversight and no further testing is required by EPA for operational use. Currently, citric acid has been cleared for general use to control tree frogs by HDOA. Pesticide Registration As a result of the caffeine tests, the Hawaii Department of Agriculture and the Department of Land and Natural Resources applied for a Federal Insecticide Fungicide and Rodenticide Act (FIFRA) Section 18 Specific Emergency Exemption to treat up to 1,200 acres with caffeine to control the tree frogs in order to protect the native biota. The exemption was granted for one year by the U.S. Environmental Protection Agency (EPA) beginning September 27, 2001. HDOA, Pesticides Branch, imposed additional specific restrictions on the use of caffeine since caffeine, a common food additive, has never been registered for use as a pesticide. Some of the restrictions have been questioned as too restrictive, e.g., requiring areas to be sealed off for 24 hours after treatment by more than just warning signs or a tape and requiring security guards or police to be posted to prevent entry and limiting the use of caffeine solely to persons certified to use restricted pesticides (not by persons under the supervision of a certified applicator). Due to these requirements, there was no operational use of caffeine to control the tree frogs for most the exemption period by either private or public sectors. Only at the very end of the exemption period was a caffeine application conducted on Maui by the Maui Invasive Species Committee (MISC) specifically to obtain efficacy and nontarget effects data that were required by EPA. The MISC application of caffeine was the first test of the efficacy of caffeine in a large-scale field application. Carribean Tree Frog Control and Eradication 5 WS has applied for a FIFRA Section 18 Quarantine Exemption to allow nursery growers to continue to use 2 percent caffeine and water solution to control frogs on plant products. The quarantine exemption allows EPA to authorize an exemption for up to 3 years. Further experimental work has been conducted by the State of Hawaii Department of Agriculture and the University of Hawaii - College of Tropical Agriculture and Human Resources, to identify a broad spectrum pesticide that can be used by the public, without a commercial pesticide license. Likely candidates are products that contain pyrenone or other synthetic pyrethroids (HDOA 2001), hydrated lime, lime sulfur and citric acid. Hydrated lime proved to be effective, but HDOA was not optimistic that hydrated lime, a soil amender, would be registered as a pesticide product, since the manufacturers that were contacted did not want to bear the heavy regulatory burden of registering the substance as a pesticide. Operations On October 1, 2002, WS and CTAHR entered into a cooperative service agreement to begin the initial phases of ISMP to suppress, control and eradicate Caribbean tree frog populations in the State of Hawaii. The planned activities covered under this agreement did not cover the full array of actions necessary to completely manage the frog problem, but it covered key components of the ISMP such as complying with the National Environmental Policy Act (NEPA), applying for a Federal Insecticide Fungicide and Rodenticide Act (FIFRA) Section 18 Quarantine Exemption to use caffeine in solution to control the frogs, conducting research to develop methods to control frogs, and to begin some operational control on the islands of 1 Kauai and Hawaii A multi-agency demonstration pilot project was implemented in December 2002 at Lava Tree State Park in Pahoa, Puna District, on the island of Hawaii. Lava Tree State Park was considered to have the highest coqui frog population density in the state. Labor crews cleared the nonnative subcanopy and ground vegetation in an area slightly less than 2 acres. The plant material was mulched and piled on site. Research crews then sprayed a 16 percent solution of citric acid to the site. Then the mulch was spread over the site. The project proved that vegetation removal was an important factor in controlling the frog numbers, and that citric acid was highly effective against frogs in a control operation. Further tests will be made to determine the efficacy of using citric acid in a control operation without clearing the vegetation. Plant Sanitation The application of hot water at 45 degrees C (113 degrees F) to plants for 3 minutes was found to be lethal to Eleutherodactylus frogs on potted tree seedlings (Arnold Hara, pers. comm). An apparatus was developed by the University of Hawaii at Hilo, the Cooperative Extension Service and the Hawaii Division of Forestry and Wildlife at the tree nursery in Hilo in which potted plants move under a conveyor belt through the hot water treatment. Frogs were killed when subjected to this hot water treatment. The treatment can be applied to commercial nursery operations. Further tests showed that vapor heat treatment used to control fruit flies in papaya, was also effective in killing tree frogs (Hara pers. comm.). Carribean Tree Frog Control and Eradication 6 A protocol for inspecting, sanitizing and modifying habitats was distributed to the nursery industry by the Hawaii Department of Agriculture. Hawaii Board of Agriculture has since declared the coqui frog an agricultural pest, thereby restricting the movement of plant products between the islands if they are infested with the frog. As recently as August 2002, two shipments of plants arriving on Oahu were found to be infested with the frogs, sealed and returned to the island of Hawaii at the shipper's expense. While the majority of plant shipments have passed inspections for the frog, at least half a dozen other plant shipments have been held on the island of Hawaii because of frog infestation (Hawaii Tribune Herald 2002). Citric acid is now being used to sanitize exported nursery plants that show no signs of phytotoxicity to the solution; but citric acid can cause leaf chlorosis to some delicate plants and flowers, however, rinsing plants with tap water after spraying minimizes damage. Alternative treatment techniques are is being sought by NWRC and CTAHR. Legal Efforts The transport of coqui frogs around the state is an illegal activity, but the law requires proof of intent to deliberately spread the frog, which is difficult to prove. In a letter to the floriculture and nursery plant industry dated June 28, 2000, the Department of Land and Natural Resources reminded the industry that spreading the frogs was illegal and asked the industry to ensure that frogs were not spread through their trade. Despite this warning, frogs are still being dispersed through the potted plant and cut flower trade, through wholesalers and retail garden outlets. 1 Information and Coordination Informational and planning meetings have been held with key officials from federal, state and county agencies and with stakeholder associations in Hawaii. An initial control plan was developed by the Hawaii Department of Agriculture and presented to the Hawaii Export Nursery Growers. A Coqui Frog Working Group was organized by the Dr. Arnold Hara of the CTAHR. The group was comprised of industry representatives and federal, state and county agency officials. The purpose of the working group was to meet regularly to share information and determine how best to control the spread of the tree frogs. The group met monthly and provided a forum for information sharing, planning operations, inspections, research, control and outreach. A number of pest alert leaflets and brochures have been produced by CTAHR and Wildlife Services. CTAHR also produced a video that was aired on the public access channel. Wildlife Services, CTAHR and the Hawaii Department of Agriculture are actively working with community and neighborhood associations to provide basic information and technical assistance in controlling frog infestations on the island of Hawaii. To date, meetings have been held with the Nanawale Community Association in Pahoa, Honokaa-Kukuihaile Community Association and the Ainaloa Community Association in Keaau. The Hawaii Department of Agriculture has purchased five 100 gal. tanks and spray pumps to loan to community groups to control the frogs. Carribean Tree Frog Control and Eradication 7 IV Life History of the Carribean Tree Frogs Hara et al. (2002) provide a life history summary of the coqui frog. The coqui frog is a native of Puerto Rico where it has been extensively studied in the field and laboratory with respect to its development, morphology, metabolism, neurophysiology and reproduction (Campbell 2001 a). The developmental biology of the coqui frog is not typical of common bullfrogs, because the fertilized egg undergo direct development rather than passing through a free- living, tadpole stage. Standing water is not required for egg-laying (Townsend and Stewart 1985, 1994). Coqui deposit 4-6 clutches of about 28 eggs each (range 16-41) per year, with a developmental period of 17-26 days, a time between clutches of about eight weeks, and a time between generations (i.e., from egg-laying adult) of about eight months (Townsend and Stewart 1994, Kraus et al. 1999). Males guard the eggs to keep them from drying out (Taigen et al. 1984) and in severely dry conditions they will leave the nest, gather moisture, and return to rehydrate the eggs (Michael 1995). In its native range, coqui frogs consume a diet consisting mostly of insects, but may include other invertebrates, such as mollusks, arachnids, centipedes and millipedes (Stewart and Woolbright 1996). Hymenopterans, primarily ants, accounted for 38 percent of the total number of prey found in coqui stomachs but made up only 6 percent of the prey volume due to their relatively small size. Orthopterans, primarily crickets and roaches, accounted for 70 percent of the total prey volume (Stewart and Woolbright 1996). Numerous invertebrates and at least 19 species of vertebrates feed on coqui frogs in its j native range. Vertebrate predator species include two frogs, three lizards, three snakes, eight birds and three mammals, with birds consuming the most frogs (Stewart and Woolbright 1996). By far the major predators on coqui eggs are the coqui themselves. Both sexes but especially males will eat coqui eggs (Townsend 1984, Townsend et al. 1984). Parental male coqui frogs may eat their own eggs if disturbed early during brooding. Egg-eating by other males is a common behavior in coqui, hence the males defend the egg clusters (Hara et al. 2002). V Carribean Tree Frog Distribution As of June 2002 Caribbean tree frogs have been reported from 327 sites occupying approximately 2,000 acres on the islands of Hawaii, Maui, Oahu and Kauai, which is .028 percent of the total land area of the state. The populations are fairly discrete. A few sites on the island of Hawaii have population densities that are high, but they occupy only a few acres. A large majority of the reported sites have a very small number of frogs (less than 10). Most of these sites are located at residences, hotels and business establishments. Most, and possibly all, populations of Caribbean tree frogs are susceptible to eradication because numbers are still low at most sites and because males can readily be targeted for removal, thereby halting reproduction (Kraus et al. 1999). Carribean Tree Frog Control and Eradication 8 Table 1. The number of verified and reported locations of Caribbean tree frogs for each island as of June 2002. Hawaii Maui Oahu Kauai Total Co ui Fro >200 40 22 6 268 Greenhouse Fro * 43 1 13 2 59 *Green house frog probably more widespread than originally reported due to their low audio detectability VI Authority for Invasive Species Management Hawaii Department of Agriculture (HDOA) The Plant Quarantine program regulates the inter-island movement of plants infested with the coqui frog under section 150A-2, Hawaii Revised Statutes (HRS), and sections 4-72-3 and 4, Hawaii Administrative Rules (HAR). Section 150A-2, HRS, defines pest as an organism that is potentially harmful to agriculture and public health or has an adverse effect on the environment as determined by the Board of Agriculture. Subsection 4-72-3(a), HAR, states that plants and propagative plant parts shall be inspected prior to transport between islands, and subsection 4-72-4(b) states that a commodity infested with a pest shall not be transported between islands unless it has been treated to exterminate the pest. The Board officially declared the coqui frog, Eleutherodactylus coqui, a pest on September 27, 2001. The Plant Pest Control Branch derives its authority to control pests from section 141-3, HRS, 1 which lists examples of pests as "insects, mites, diseases, noxious weeds, or other pests injurious to vegetation of value." The program's authority to control or eradicate pests is further defined in chapter 4-69A, HAR. Section 4-69A-2, HAR, defines other pests as any invertebrate that is harmful to agriculture or vegetation of value. Based on this definition, the Plant Pest Control program does not have authority to control or eradicate frogs or other vertebrate animals, nor does it have the authority to enter private property to control or eradicate these animals. Importing states and countries determine whether an organism is regulated as a pest on commodities exported out of the State. No other entities have declared the frogs as pests, thus far. College of Tropical Agriculture and Human Resources, University of Hawaii (CTAHR) The College of Tropical Agriculture and Human Resources is an integral part of the University of Hawaii at Manoa's Carnegie I Research Institution designation and is the Land Grant College of the University of Hawaii and Hawaii. CTAHR is federally mandated to fulfill the University's threefold Land Grant mission of instruction, scientific research and outreach to address State needs. The Morrill Land Grant College Act of 1862 initiated giving public land to each state to endow a college that emphasized practical education in agriculture and engineering. The Hatch Act of 1887 provided funds to each state agricultural college to operate an experiment station that promotes the use of scientific research to solve agricultural problems. In fulfillment of these acts, The Hawaii Agricultural Experiment Station was established in 1901. Six years later (1907), the College of Agriculture and Mechanical Arts of the Territory of Hawaii was founded, marking the beginning of the University of Carribean Tree Frog Control and Eradication 9 Hawaii. Through a cooperative agreement with the United States Department of Agriculture, Cooperative States Research, Education and Extension Service, the University of Hawaii receives funding through the Special Grants Program. The Tropical and Subtropical Agricultural Research Program (TSTAR) Special Research Grant is administered through regulations established by the following federal regulation, primarily 7 CFR Parts 3015 and 3019 Uniform Administrative Requirements for Grants and Other Agreements With Institutions of Higher Education, Hospitals, and Other Nonprofit Organizations" . Animal and Plant Health Inspection Service, Wildlife Services (APHIS-WS) The Wildlife Services program of the Animal and Plant Health Inspection Service, U.S.. Department of Agriculture is the Federal agency authorized to manage wildlife that threaten natural resources, agriculture and human health and safety. The primary authority WS is the Animal Damage Control Act of March 2, 1931, as amended 46 Stat. 1468; 7 U.S.C.426-426b and 426c), and the Rural Development, Agriculture, and Related Agencies Appropriations Act of 1988 (P.L. 100-102) and the FY 2001 Agriculture Appropriations bill. Department of Land and Natural Resources (DLNR) All species in the Order Anura are listed as Injurious Wildlife in HRS Title 13 Chapter 124. This designation makes it unlawful for anyone to release these organisms into the wild, transport them to areas where they are not currently established, or export, them from the state. Exceptions are granted.via a DOFAW permit for a one-time personal use export or export to a known research institution. U.S. Fish and Wildlife Service (FWS) The Lacey Act (18 U.S.C. 42), administered by the U.S. Fish and Wildlife Service, prohibits importation into the United States or any U.S. territory or possession and shipment between the continental United States, the District of Columbia, Hawaii, the Commonwealth of Puerto Rico, and any possession of the United States of certain categories of animal species determined to be "injurious to human beings, to the interests of agriculture, horticulture, forestry, or to wildlife or the wildlife resources of the United States." Wildlife and wildlife resources are defined broadly to include all wild animals and "all types of aquatic and land vegetation upon which such wildlife resources are dependent." Id. § 42(a)(1). The statute gives the FWS the,authority to export or destroy any injurious species at the expense of the importer, id., although permits may be issued to allow importation of otherwise injurious species for specific purposes, id. § 42(a)(3). Regulations listing species found to be injurious under the Lacey Act are in 50 CFR parts 16. VII Compliance with Federal Laws and Court Orders Several Federal laws regulate wildlife damage management. The following Federal laws are relevant to the actions considered: National Environmental Policy Act Environmental documents pursuant to NEPA must be completed before actions can be implemented. NEPA requires that Federal actions be evaluated for environmental impacts, that these impacts be considered by the decision maker(s) prior to implementation, and that the public be informed. Carribean Tree Frog Control and Eradication 10 Endangered Species Act. It is Federal policy, under the ESA, that all Federal agencies shall seek to conserve endangered and threatened species and shall utilize their authorities in furtherance of the purposes of the ESA (Sec.2(c)). Section 7 consultations with the USFWS are conducted to use the expertise of the USFWS to ensure that "any action authorized, funded, or carried out by such an agency.... is not likely to jeopardize the continued existence of any endangered or threatened species. Each agency shall use the best scientific and commercial data available" (Sec.7(a)(2)) Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA). FIFRA requires the registration, classification, and regulation of all pesticides used in the United States. The Environmental Protection Agency (EPA) is responsible for implementing and enforcing FIFRA. All chemical methods integrated into any selected program as implemented by APHIS-WS or other cooperating agencies must be registered with and regulated by the EPA and the HDOA, and used' in compliance with labeling procedures and requirements. Animal Damage Control Act and the Rural Development, Agriculture, and Related Agencies Appropriations Act. The Acts authorize and direct APHIS-WS to reduce damage caused by wildlife in cooperation with other agencies. Protection of Children from Environmental Health and Safety Risks (EO13045). Children may suffer disproportionately from environmental health and safety risks for many reasons. The Carribean tree frog eradication plan as proposed would only involve legally available and approved damage management methods in situations or under circumstances where it is highly unlikely that children would be adversely affected. Therefore, implementation of the proposed action would not increase environmental health or safety risks to children. Impacts on minority and low income persons or populations (Environmental Justice and Executive Order 12898) EO 12898 requires Federal agencies to make Environmental Justice part of their mission, and to identify and address disproportionately high and adverse human health and environmental effects of Federal programs, policies and activities on minority and low income persons or populations. All activities are evaluated for their impact on the human environment and compliance with EO 12898 to ensure Environmental Justice. Invasive Species (EO 13112). The Invasive Species Executive Order directs Federal agencies to use their programs and authorities to prevent the spread or to control populations of invasive species that cause economic or environmental harm, or harm to human health. Lacey Act (16 U.S.C. 3371 et seq.) The Lacey Act has implications for regulating introductions of invasive species. This law, administered by the Secretaries of the Interior, Commerce, and Agriculture, generally makes it unlawful for any person to import, export, transport, sell, receive, acquire, or purchase (or attempt to commit any such act) in interstate or foreign commerce any fish, wildlife, or plant taken, possessed, transported, or sold in violation of any Federal, tribal, State, or foreign law. !d. § 3372 (a)(1), (2), (4). Thus, while the statute does not substantively grant authority to regulate the importation, transportation, exportation, or possession of any species, violation of another Federal, State, tribal, or foreign law governing these activities would become a violation of Federal law and subject to Carribean Tree Frog Control and Eradication 11 particular civil and criminal penalties. See id. 3373, 3374. The Secretaries of the Interior and Commerce have the authority to enforce laws involving fish and wildlife, while the Secretary of Agriculture has the authority to enforce laws involving plants. Texas FOIA Decision The U.S. District Court in Waco, Texas issued a judgment on September 30, 2002, enjoining APHIS-WS from releasing any personal identifying information in violation of the Freedom of Information Act and Privacy Act. This decision has implications to WS NEPA procedures. Names and locations of cooperating agencies and actions will be redacted from any APHIS-WS NEPA document when presented to the public. International Agreements and Authorities The World Trade Organization (WTO) Agreement on the Application of Sanitary and Phytosanitary (SPS) Measures, 1995. The SPS Agreement is a supplement to the World Trade Organization Agreement. It provides a uniform framework for measures to protect the health and lives of humans, plants, and animals. Sanitary and phytosanitary measures are defined as actions whose goal is to: 1) protect human, animal, or plant health from the entry or spread of pests, disease, or disease carrying organisms; or 2) prevent or limit other damage from the entry or spread of pests. The SPS Agreement has chosen the international standards, guidelines, and recommendations of three organizations - International Plant Protection Convention (IPPC), Codex, and Office International des Epizooties (OIE) as the preferred measures for adoption by WTO members South Pacific Regional Environment Program (SPREP) Convention, 1990 Article 14 states that the Parties shall take measures to protect rare or threatened ecosystems and species within the region. In 1999, SPREP produced an Invasive Species Strategy for the Pacific Islands Region. The Strategy promotes efforts of Pacific Island countries to protect their natural heritage from the impacts of invasive species through cooperative efforts and capacity building. VIII Hawaii State laws Hawaii Revised Statutes Chapter 124 Injurious Wildlife All species in the Order Anura are listed as Injurious Wildlife in HRS Title 13 Chapter 124. This designation makes it unlawful for anyone to release these organisms into the wild, transport them to areas where they are not currently established, or export, them from the state. Exceptions are granted via a DOFAW permit for a one-time personal use export or export to a known research institution. Hawaii Revised Statutes Chapter 150A Plant and Non-Domestic Animal Quarantine "Hawaii Plant Quarantine Law" gives the Department of Agriculture responsibility to keep the State free of imported agricultural pests and allow the department to receive gifts to assist in preventing entry of brown tree snakes into the State. Carribean Tree Frog Control and Eradication 12 Hawaii Revised Statutes Chapter 343 Environmental Impact Statements Hawaii's EIS law requires an environmental assessment be prepared for certain actions. The actions that are being analyzed in this EA would typically be subject to Chapter 343 EA requirement if they are done within the following land designations: 1) any land classified as conservation district by the state land use commission, 2) any shoreline management areas as defined in section 205A-41, 3) any historic site as designated in the National Register or Hawaii Register as provided fro in the Historic Preservation Act of 1966, PL 89-665, or chapter 6E. However, according to DLNR, controlling frogs can be carried out on state lands without an EA, as defined in section 5 Part 5 of the DOFAW approved exemption list. This exemption applies when the management activity takes place over relatively small areas. IX Carribean Tree, Frog Control and Eradication Campaign The distribution of Carribean Tree Frogs is increasing but they remain in fairly discrete populations that can be eliminated if aggressive action is undertaken immediately. An additional delay of one or two years may close the window of opportunity to eradicate these invasive species from Hawaii. Elements of the frog control and eradication campaign are presented below. 1. Enforce current rules that prohibit the spread of frogs to other parts of the state. Responsible agency: Hawaii Department of Land and Natural l Resources (HDLNR) The transport of frogs around the state is an illegal activity. Current Department Land and Natural Resources rules establish that the Eleutherodactylus frogs are injurious wildlife and make it illegal to 1) release injurious wildlife into the wild; 2) transport them to islands or locations within the state where they are not already established and living in a wild state; 3) export any such species, or the dead body or parts thereof, from the state (Hawaii Administrative Rules, Title 13, Subtitle 5, Chapter 124, Section 3d). Action: Assign state officials to work with the plant industry and public to stop the spread of the frog and enforce current HDLNR rules. 2. Establish a quarantine, treatment and certification program for movement of plants from commercial nurseries. Responsible agencies: Hawaii Department of Agriculture (HDOA) Action: Continue implementation of a quarantine of all infested plant material. Develop better treatment and certification protocols through compliance agreements with commercial plant nurseries. Increase the number of inspectors on the island of Hawaii to enforce compliance and to inspect plant material being moved through non-commercial sources through interisland ports. Identify and register more pesticide products that are available to nurseries for use in the control of frogs. Carribean Tree Frog Control and Eradication 13 I 3. Conduct research to develop control methods. Responsible agencies: National Wildlife Research Center (NWRC) and University of Hawaii College of Tropical Agriculture and Human Resources (CTAHR) Action: Continue development of cultural, mechanical, chemical, and bio-control techniques to control frogs in the wild and on nursery products. 4. Inform and educate the general public, industry and government leaders on the problems caused by the frogs and the need to stop the spread of the frog and how to implement control measures on incipient and established populations. Responsible agencies: WS, HDOA, CTAHR Action: Develop a variety of media to inform the general public, neighborhood associations and community groups, commercial nursery growers, and retail outlets about the frog problem and how to control populations. 5. Prepare environmental analysis of proposed frog eradication campaign to comply with the National Environmental Policy Act (NEPA). Responsible agency: Wildlife Services (WS) Action: NEPA document in progress. Anticipated issues in implementing the ISMP is the impact to humans and secondary hazards to the environment. 6. Implement the control of incipient populations in cooperation with neighborhood and community associations, state and county agencies. (Oahu, Maui, Kauai, Hawaii). Responsible agencies: WS, NWRC, HDOA, HDLNR, Counties Action: Control incipient populations of Caribbean tree frogs on Kauai, Maui, Oahu and Hawaii. USDA-Wildlife Services has the legal mechanism to work on private residential and commercial property with the owner's permission. Sites will be prioritized and selected based on consultations by WS and cooperating agencies, neighborhood boards, community groups and associations, and industry. Wildlife Services will coordinate with state and county officials and interested groups to initiate treatment plans. Operations will be conducted based on ease of access, proximity to sensitive areas, state, county or owner approvals, and available resources. After every operation, WS and NWRC will evaluate treatment efficacy and determine whether follow-up actions will be needed. As populations are eliminated, resources will be shifted where needed. 7. Implement control of high density populations with control teams (Island of Hawaii). Responsible agencies: Wildlife Services, NWRC, HDOA, CTAHR, HDLNR, County of Hawaii USDA-Wildlife Services, HDOA and CTAHR will work with nursery growers that Carribean Tree Frog Control and Eradication 14 are known or suspected sites of frog dispersal through the commercial plant trade. Growers would be required, through a quarantine program, to treat infested plant products using available methods to obtain frog free certification. USDA-Wildlife Services would assist in treating areas surrounding commercial operations with the permission of the landowners. The purpose of assisting commercial operations in the control of frogs is to reduce the chance of dispersal through commercial plant products. USDA-Wildlife Services will work with federal, state and county agencies on public lands having high densities of frogs. The areas occupied will be identified and pesticides applied to stop the spread of the frog. The action may require the clearing of vegetation to provide better access for treatments, and will be the responsibility of the appropriate land management agency. After every operation, Wildlife Services and NWRC will evaluate the potential secondary impacts, and determine if actions are needed to improve efficiency and effectiveness of kill. v Carribean Tree Frog Control and Eradication 15 Table of Cost Estimates Element (lead agency) FY 2004 FY 2005 FY 2006 NEPA Compliance (USDA) $350,000 Enforcement (HDLNR) $150,000 $150,000 $150,000 Certification and Treatment $300,000 $300,000 $300,000 Compliance (HDOA) Research (NWRC, CTAHR) $500,000 $500,000 $500,000 Information/Education, Community $200,000 $200,000 $200,000 Action (HDOA) Control Incipient Populations on $915,000 $951,600 $980,200 Oahu, Kauai, Maui (USDA) Control High Populations on the $1,459,800 $1,503,594 $1,548,700 island of Hawaii (USDA) TOTAL $3,874,800 $3,605,194 $3,678,900 Carribean Tree Froa Control and Eradication X Interagency Coordination and Public Involvement Personnel from the following agencies and companies have been consulted or participated in the development or review of this plan. William Pitt, Ph.D., Research Scientist Robert Sugihara USDA APHIS WS National Wildlife Research Center Hawaii Field Station Larry Nakahara, Manager Kyle Onuma, Entomologist Plant Pest Control Branch Division of Plant Industry Hawaii Department of Agriculture Arnold Hara, Entomologist Cooperative Extension Service University of Hawaii College of Tropical Agriculture and Human Resources 1 Mike E. Pitzler, State Director Tim Ohashi Shayne Veriato Hawaii Guam and Pacific Islands USDA APHIS WS Lyle Wong, Ph.D., Administrator Division of Plant Industry Hawaii Department of Agriculture Carribean Tree Frog Control and Eradication 17 VI Bibliography Campbell, E.W. 2001 a. Dermal toxicity of selected agricultural pesticides, pharmaceutical products, and household chemicals to introduce Eleutherodactylus frogs in Hawaii - Draft NWRC, USDA-APHIS-WS, Fort Collins, CO. Campbell, E.W. 2001b. Field efficacy trials of the directed spray application of caffeine solutions for controlling introduced Eleutherodactylus frogs in flouriculture and nursery crops in Hawaii - Draft. NWRC, USDA-APHIS-WS, Fort Collins, CO. Hawaii Agriculture Statistics Service (HASS). 2000. Statistics of Hawaii Agriculture 1999. Honolulu HI Hawaii Export Nursery Association. 2001. Proposed Plan for Controlling the Spread of Caribbean Tree Frogs. Hawaii Department of Agriculture. 2001. Pesticide Trials for Coqui Frogs. August 20, 2001, Plant Industry Division, Honolulu HI Hawaii Department of Agriculture and Hawaii Department of Land and Natural Resources. 2001. Request for Specific Exemption Under Section 18 of FIFRA to Allow Field Use of Caffeine to Control the Coqui and Greenhouse Frogs in Floriculture and Nursery Crops, Residential Areas, Parks, Hotels, Resorts and Forest Habitats in the State of Hawaii. Kraus, F., E.W. Campbell, A. Allison, and T. Pratt. 1999. Eleutherodactylus frog introductions in Hawaii. Herp. Rev. 30:211-25 Carribean Tree Frog Control and Eradication 18 Verified and reported Carribean frog (Eleutherodactylus spp.) locations on the Island of Hawaii Hawl P 't« S v '1~X~ d'tt c t _ , Sh * Y f H ~ ^S Hllo s ~ ~ 'F^Mx ~,,?i espy • ge, t ` Kailua-Kona ar 1954 34w i Pahoa ~_v n Naalehu r M 1 ® E. coqui (n = >200) ® E. planirostris (n = 43) Major Roads N Updated June 2002 30 s~ Kilometers Hawaii Field station National Wildlife Research Center USDA/APHIS-WS Hilo, Hawaii Verified and reported Carribean frog (Eleutherodactylus spp.) locations on the Island of Oahu r. ti ~k 1~ 1 f ~ g 'd 1~4 r k i ~ f Y 4y.~ g (j)f ~ rz 1 17 r ~ r ~s~ N ? " R Kailua jj 4' t r ~ 4 T -'G ~ , r * t C y a+er r « a fr • Kapolei Honolulu it E. coqui (n = 22) ® E. planirostris (n =13) N Major Roads 10 0 10 20 Kilometers Updated June 2002 Hawaii Field Station National Wildlife Research Center USDA/APHIS-WS Hilo, Hawaii All verified and reported Caribbean frog ' (Eleutherodactylus spp.) locations on the Island of Kauai PRINCEVILLE ; ull igg" r kV~o nt f t!'~ ~jyL WAILUA rr ~xts o r "k, Eve ~ r bA I r pa f~ .:-tr *T 'LIHUE J47 I ELEELE POIPU • E. coqui (n = 6) A E. planirostris (n = 2) Major roads L~ N 10 0 10 Kilometers Updated June 2002 USDA - National Wildlife Research Center Hawaii Field Station Hilo, Hawaii Eleutherodactylus Frog Eradication - State of Hawaii Invasive Species Management Plan l Prepared by: United States Department of Agriculture Animal and Plant Health Inspection Service Wildlife Services 3375 Koapaka Street, Suite H420 Honolulu, Hawaii 96819 ph. 808 861-8577 fax 808 861-8570 Email: mike.e.pitzler(o)usda.gov Hawaii Department of Agriculture Division of Plant Industry 1428 S. King Street Honolulu, Hawaii 96814 ph. 808 973-9535 fax. 808 973-9533 Email: lwongpi(LDyahoo. corn Ver. 8/25/01 I Summary Proposed Action: Invasive Eleutherodactylus Frog Eradication in the State of Hawaii Lead State Agency: State of Hawaii Department of Agriculture Plant Industry Division 1428 South King Street Honolulu, Hawaii 96814 State Official: Lyle Wong, Ph.D. Administrator, Plant Industry Division ph. 808 973-9530, fax 808 973-9533 Email: Iwongpi@yahoo.com Lead Federal Agency: U.S. Department of Agriculture Animal and Plant Health Inspection Service Wildlife Services 3375 Koapaka Street, H 420 Honolulu, Hawaii 96819 ( Federal Official: Mike E. Pitzler, \ State Director Hawaii/Pacific Islands USDA APHIS WS ph. 808 861-8577, fax 808 861-8570 Email: mike.e.pitzler(usda.gov For Further Information Contact: Tim J. Ohashi Program Wildlife Biologist USDA APHIS WS P.O. Box 786 Volcano, HI 96785-0786 Ph. 808 985-7275 Email: tohashi@lava.net Wildlife Services and its cooperators the Hawaii Department of Agriculture and the University of Hawaii Cooperative Extension Service, College of Tropical Agriculture and Human Resources, are proposing to implement an emergency Eleutherodactylus frog eradication campaign within the State of Hawaii. The coqui frog (Eleutherodactylus coqui) and greenhouse frog (E. planirostris) are considered to be invasive pests that have the potential to severely impact floriculture and plant nursery products industry, other private commerce, native ecosystems, and the quality of life in Hawaii and other Pacific Islands. In early 1998, eight occurrences were reported, but two and a half years later, over 85 occurrences were documented and another 65 were reported but unconfirmed. The coqui frog is now firmly established at about 150 sites on the island of Hawaii and about 40 sites on Maui with a growing number of sites on Oahu. The tree frogs can reach densities greater than 20,000 individuals per hectare. The calls of male ooqui in such high numbers can be a severe nuisance problem. The distribution of Eleutherodactylus frogs is increasing but they remain in discrete populations that can be eliminated if aggressive action is undertaken immediately. An additional delay of one or two years may close the window of opportunity to eradicate these invasive species from Hawaii. Wildlife Services and the Hawaii Department of Agriculture have prepared an Invasive Species Management Plan to Eradicate Eleutherodactylus Frogs in the State of Hawaii. The campaign would involve strict enforcement of existing state rules; quarantine, treatment and certifcafion of plant products, research to develop control methods, public information, control of high densities of frogs on the island of Hawaii and Maui and eradication of incipient populations on Kauai and Oahu. The plan would begin in fiscal year 2001 and continue for an additional three year period. Research has identified an aqueous caffeine solution that is highly effective in killing frogs. The Hawaii Departments of Agriculture and Land and Natural Resources have applied for a Federal Insecticide Fungicide and Rodenticide (FIFRA) Section 18 specific emergency exemption to use the solution to control the frogs in Hawaii. The application is being reviewed by U.S. Environmental Protection Agency. Tests are also being conducted on broad spectrum pesticides containing ti... synthetic pyrethroids that have shown promise in killing the frogs. Wildlife Services would play a lead federal role in the eradication campaign, working closely with the Hawaii Department of Agriculture and other agencies, the floriculture and nursery products industry and community groups. Wildlife Services has begun the process of complying with National Environmental Policy Act requirements. I Introduction This Invasive Species Management Plan (ISMP) was developed to eradicate Eleutherodactylus frogs in the State of Hawaii. It incorporates a previous plan for controlling the frogs that was produced by an ad hoc working group consisting of the Hawaii Department of Agriculture, Hawaii Pest Control Association, Wildlife Services National Wildlife Research Center, hotel industry representatives and the County of Hawaii (Hawaii Export Nursery Association 2001, see Appendix 1). This ISMP was further developed after discussions and reviews by the County of Hawaii, the University of Hawaii Cooperative Extension Service, PBR Hawaii, the Hawaii Department of Agriculture, the National Wildlife Research Center and industry representatives. The Eleufherodactylus frogs are native to the Caribbean and have recently become established as an alien invasive species in the State of Hawaii. The coqui frog (Eleufherodactylus cogw) and greenhouse frog (E. planirostris) are considered to be pests that have the potential to severely impact agriculture, private commerce, native ecosystems and the quality of life in Hawaii and other Pacific Islands (Campbell 2001). The U.S. Department of Agriculture (USDA) is authorized by law to protect American agriculture and other resources from damage and conflicts associated with wildlife. The primary authority for the USDA Animal and Plant Health Inspection Service (APHIS) Wildlife Services (WS) program is the Animal Damage Control Act of March 2, 1931, as amended (46 Stat. 1468; 7 U.S.C.426-426b and 426c) and the Rural Development, ( Agriculture and Related Agencies Appropriations Act of 1988 (P.L. 100-102). Executive Order 13112 further \ directs Federal agencies to use their programs and authorities to prevent the spread or to control populations of invasive species that cause economic, ecological, and human health impacts. All WS activities are conducted in cooperation with other federal, state, and local agencies, and private organizations and individuals. II Background Eleutherodactylus coqui and E. planirostris are two relatively recent invasive species introductions to Hawaii (Kraus, et.al. 1999) which does not have any native amphibians or reptiles. Unconfirmed reports of one or more of Eleutherodactylus frogs were first reported in Hawaii as early as 1988 (Kraus et. al. 1999). Both frogs are native to the Caribbean, although one or both have become established in the states of Florida, Louisiana, and Alabama (Kraus et.al. 1999). The frogs are believed to have entered Hawaii on contaminated nursery plants. In early 1992, there were only sporadic sightings of Eleutherodactylus frogs on the island of Hawaii. In early 1998, eight occurrences were reported, but two and half years later, over 85 occurrences were documented and another 65 were reported but unconfirmed (HDOA and HDLNR 2001). Eleutherodactylus coqui is now firmly established at about 150 specific sites on the island of Hawaii and at about 40 sites on Maui (Appendix 2-5). Eleutherodactylus planirostris is reported at a number of sites on Kauai, Oahu, Maui and Hawaii. The established sites for both frogs include commercial plant nurseries, retail garden outlets, residential areas, resorts and hotels, public parks and sites within forests. One of the most impressive concentrations of Eleutherodactylus frogs occurs at the entrance of Lava Tree State Park near Pahoa Village where the frog densities are high and the vocalizations of males has been described as deafening. The frogs are being spread to additional sites on infested plant material primarily from commercial nurseries and greenhouses from East Hawaii island (Kraus et.al. 1999). In addition, there are reports of deliberate frog dispersal by individuals (HDOA and HDLNR 2001). High densities of Eleutherodactylus frogs in residential areas have become a noise nuisance due to the male's loud calls which can reach 90 decibels. Vendor and public concern over purchasing plant material infested with frogs is beginning to have serious adverse impacts on the floriculture and nursery products industry. Grower sales of Hawaii's floriculture and nursery products totaled a record $75.4 million in 1999. Hawaii island, with $41.6 million in sales, ranked number one in 1999 with most of the nurseries in East Hawaii (HASS 2000) There is great concern that these frogs pose a threat to the native forest ecosystem, including many endangered species. Eleutherodactylus frogs have the potential to be a serious threat to native insects (Earl Campbell pers. comm.) and birds (Kraus et al. 1999). An analyses of the stomach contents of Eleutherodactylus frogs collected on the island of Hawaii indicate that they consume native insects (E. Campbell pers comm). Eleutherodactylus frogs could, therefore, exert tremendous predation pressure on a wide variety of native arthropods, many of which may already be stressed to the edge of extinction due to the establishment of other non-native predators and parasitoids (Kraus et al. 1999). The majority of native birds are partially or wholly insectivorous. Eleutherodactylus frogs could indirectly effect some populations of these birds (HDOA and HDLNR 2001) if the frogs spread to native forest bird ranges. None of the known sites are inhabited by endangered species but there is potential for the frogs to enter areas where threatened or endangered species are present (HDOA and HDLNR 2001). In its native range on Puerto Rico, coqui populations can reach densities greater than 20,000 individuals per hectare and consume an estimated 114,000 prey per night per hectare (Kraus et al. 1999). Since Hawaii's climate is similar to that of Puerto Rico, it is expected that Eleutherodactylus frog densities will also reach these densities in Hawaii (HDOA and HDLNR 2001). At one collection site on the island of Hawaii, 105 frogs were collected from a 50 m2 area in 50 minutes (Kraus et al. 1999). Based on this collection rate, the population estimate for this site is believed to be ten times that reported for coqui in unmanipulated native rainforests in Puerto Rico (Kraus et al 1999). Actions Taken Laboratory tests were conducted to evaluate various chemicals for use in controlling Eleutherodactylus frogs (Campbell 2001 a). The study found that dermal exposure to caffeine and water caused 90% or greater mortality to both the frogs. Field efficacy trials subsequently were conducted to test the application of caffeine solutions for controlling the frogs in floriculture and nursery crops in Hawaii (Campbell 2001 b). These trials showed that a single. spray application of 2 percent concentration caffeine solution caused a 100% decline in the relative abundance of Eleutherodactylus frogs (Campbell 2001 b). Concentrations down to 0.5 percent were tested with similar success depending on the density and type of foliage (HENA 2001). As a result of the caffeine tests, the Hawaii Department of Agriculture and the Department of Land and Natural Resources have applied for a Federal Insecticide Fungicide and Rodenticide Act (FIFRA) Section 18 Specific Emergency Exemption to treat up to 1,200 acres with caffeine to control the Eleutherodactylus frogs in order to protect the native biota (Appendix 6). The application was sent to the U.S. Environmental Protection Agency on June 1, 2001. If the Emergency Exemption is granted, the authority for use will be for one year. It is expected that EPA will request additional data to support the use of the caffeine solution beyond the one year period. The application may be amended to request a quarantine exemption to protect the floriculture and nursery plant industries which are beginning to experience negative economic impacts from the sale of infested plant material. Eleutherodactylus Frog Eradication Plan 3 Experimental work is also being conducted by the State of Hawaii Department of Agriculture and the University of Hawaii - College of Tropical Agriculture and Human Resources, to identify a broad spectrum pesticide that can be used by the public, without a commercial pesticide license. Likely candidates are products that contain pyrenone or other synthetic pyrethroids (HDOA 2001). The use of such products may be applicable to nurseries and wild areas adjacent to nurseries where the frogs have become established in dense colonies. Additional work is required to further evaluate additional chemicals to control the frogs. The application of hot water at 45 degrees C (113 degrees F) to plants for 3 minutes was found to be lethal on Eleutherodactylus frogs on potted tree seedlings (Arnold Hara, pers. comm. An apparatus was developed by the University of Hawaii at Hilo, the Cooperative Extension Service and the Hawaii Division of Forestry and Wildlife at the tree nursery in Hilo in which potted plants move under a conveyor belt through the hot water treatment. The treatment can be applied to commercial nursery operations. The transport of frogs around the state is an illegal activity. In a letter to the floriculture and nursery plant industry dated June 28, 2000, the Department of Land and Natural Resources reminded the industry that spreading the frogs was illegal and asked the industry to ensure that frogs were not spread through their trade. Despite this warning, frogs are still being dispersed through the potted and cut plant trade. Informational and planning meetings have been held with key officials from federal, state and county agencies and with stakeholder associations in Hawaii. An initial control plan was developed by the Hawaii Department of Agriculture and presented to the Hawaii Export Nursery Growers. A protocol for inspecting, sanitizing and modifying habitats was distributed to the nursery industry by the Hawaii Department of Agriculture (Appendix 7). Hawaii Department of Agriculture is considering the implementation of a quarantine of plant products that are infested with frogs. There is virtually no control.or management of the frogs being implemented by either government agencies or the industry. Frogs are being caught by hand at sites on Oahu and Maui by volunteers. Hand capture can be an effective method of controlling male coqui frogs when they are first detected in anew area. While females are more difficult to control because they do not vocalize, the removal of males will suppress breeding potential. A large-scale and site specific control program would require the development of control techniques and the design of an integrated pest management system to control and eradicate the frogs. III Eleutherodactylus Frog Eradication Campaign The distribution of Eleutherodactylus frogs is increasing but they remain in discrete populations that can be eliminated if aggressive action is undertaken immediately. An additional delay of one or two years may close the window of opportunity to eradicate these invasive species from Hawaii. Elements of an Eleutherodactylus frog eradication campaign are presented below. M. 1. Enforce current rules that prohibit the spread of frogs to other parts of the state. r~ Responsible agency: Hawaii Department of Land and Natural Resources (HDLNR) Eleutherodactylus Frog Eradication Plan 4 The transport of frogs around the state is an illegal activity. Current Department Land and Natural Resources rules establish that the Eleutherodactylus frogs are injurious wildlife and make it illegal to 1) release injurious wildlife into the wild; 2) transport them to islands or locations within the state where they are not already established and living in a wild state; 3) export any such species, or the dead body or parts thereof, from the state (Hawaii Administrative Rules, Title 13, Subtitle 5, Chapter 124, Section 3d). Action: Assign state officials to work with the plant industry and public to stop the spread of the frog and enforce current HDLNR rules. 2. Establish a quarantine, treatment and certification program for movement of plants from commercial nurseries. Responsible agencies: Hawaii Department of Agriculture (HDOA) Action: Implement a quarantine of all infested plant material. Develop treatment and certification protocols through compliance agreements with commercial plant nurseries. Increase the number of inspectors on the island of Hawaii to enforce compliance and to inspect plant material being moved through non-commercial sources through interisland ports. Identify and register existing pesticide products that are available to nurseries for use in the control of frogs. 3. Conduct research to develop control methods. Responsible agencies: National Wildlife Research Center (NWRC) Action: There are currently no cost-effective techniques to treat frog infestedsites on a large-scare. Similarly, there are no techniques commerciallyavailable to treat frog infested plant material. There is an urgent needto rapidly develop cost-effective techniques to assist WS Operations andother entities with the control of tree frogs. Research on biologicallysound cultural, mechanical, chemical, and bio-control techniques needs toexpanded or initiated immediately. In addition to in-house research, NWRCstaff (in coordination with local agencies) will develop cooperativeagreements with local research facilities to conduct collaborativeresearch. 4. Inform and educate the general public, industry and government leaders on the problems caused by the frogs and the need to stop the spread of the frog and how to implement control measures on incipient and established populations. Responsible agencies: HDOA, CTAHR Action: Develop media to inform the general public, community groups, educational institutions and stakeholders about the frog problem. A video is necessary and will be a valuable teaching aid when snaking presentations to community groups and will be in a ems, format suitable for television programming. Radio announcements will be produced and aired on the island of Hawaii to inform the public in an effort to prevent the deliberate spread of the frog to other parts of the island and the state. Neighborhood Eleutherodactylus Frog Eradication Plan 5 watch groups will be assisted by agencies for early detection and control of frogs. The existing Big Island Invasive Species Committee alien species hotline and GIS record- keeping will be supported. 1. Prepare environmental analysis of proposed frog eradication campaign to comply with the National Environmental Policy Act (NEPA). Responsible agency: Wildlife Services (WS) Action: NEPA document in progress. Anticipated issues in implementing the ISMP is the impact to humans and secondary hazards to the environment. 6. Implement the control of incipient populations through use of control teams and industry cooperation (Oahu, Maui, Kauai). Responsible agencies: WS, NWRC, HDOA, HDLNR, Counties Action: Using hand capturing, cultural methods, aqueous caffeine solution or other available chemical products, control would be implemented immediately on incipient populations of Eleutherodactylus frogs on Oahu, Maui and Kauai. USDA-Wildlife Services has the legal mechanism to work on private property with the owners permission. Sites will be prioritized and selected based on priorities developed by WS, I" HDOA, and the counties. Wildlife Services will coordinate with state and county officials and industry groups to initiate treatment plans. Operations will be conducted based on ease of access, proximity to sensitive areas, state, county or owner approvals, and available resources. After every operation, USDA Wildlife Services and NWRC will evaluate the frog kill, secondary effects, and determine whether follow-up actions will be needed. As populations are eliminated, resources will be shifted where needed. 7. Implement control of high density populations with control teams (Island of Hawaii). Responsible agencies: Wildlife Services, NWRC, HDOA, CTAHR, HDLNR, County of Hawaii USDA APHIS WS may apply for a separate FIFRA Section 18 Quarantine Exemption to allow use of an aqueous caffeine solution specifically and solely for WS operational personnel. Using this caffeine solution, hot water or other available pesticide products, control would be implemented immediately on incipient populations of Eleutherodactylus frogs on the island of Hawaii. USDA-Wildlife Services, HDOA and CTAHR will work with nursery growers that are known or suspected sites of dispersal through the commercial plant trade. Growers would be required, through a quarantine program, to treat infested plant products, either with heat treatment (heating potted plants to 45 degrees Celsius for 3 minutes), an aqueous caffeine solution, or with other approved pesticide products to obtain frog free certification. USDA-Wildlife Services would assist in treating areas surrounding commercial operations with the permission of the landowners. Eleutherodactylus Frog Eradication Plan 6 USDA-Wildlife Services will work with federal, state and county agencies on public lands having high densities of frogs. The areas occupied will be identified and pesticides applied to stop the spread of the frog. The action may require the clearing of vegetation to provide better access for treatments, and will be the responsibility of the appropriate land management agency. After every operation, Wildlife Services and NWRC will evaluate the frog kill, secondary effects, and determine if actions are needed to improve efficiency and effectiveness of kill. IV Table of Cost Estimates Element (lead agency) FY 2001 FY 2002 FY 2003 FY 2004 Enforcement(HDLNR) $10,000 $75,000 $75,000 $75,000 Certification and Treatment Compliance $10,000 $300,000 $300,000 $300,000 (HDOA) Research (NWRC, CTAHR) $50,000 $550,000 $550,000 $550,000 InfonnationlEducation, Community Action $25,000 $200,000 $200,000 $200,000 (HDOA) Control Incipient Populations on $50,000 $915,000 $951,600 $980,200 Oahu, Kauai, Maui (USDA) Control High Populations on the island of $50,000 $1,459,800 $1,503,594 $1,548,700 Hawaii (USDA) TOTAL $206,000 $3,584,800 $3,580,194 $3,653,900 V Interagency Coordination and Public Involvement Personnel from the following agencies and companies have been consulted or participated in the development or review of this plan. Eleutherodactylus Frog Eradication Plan 7 1 Earl Campbell III, Ph.D., Research Scientist USDA APHIS WS National Wildlife Research Center Hawaii Field Station Larry Nakahara, Manager Kyle Onuma, Entomologist Plant Pest Control Branch Division of Plant Industry Hawaii Department of Agriculture Arnold Hara, Entomologist Cooperative Extension Service University of Hawaii College of Tropical Agriculture and Human Resources Yukie Ohashi, Environmental Planner (Volunteer) PBR Hawaii Mike E. Pitzler, State Director Hawaii Guam and Pacific Islands USDA APHIS WS Margarita Hopkins, Director Research and Development County of Hawaii Lyle Wong, Ph.D., Administrator Division of Plant Industry Hawaii Department of Agriculture Plan Prepared By Tim J. Ohashi, Program Wildlife Biologist U.S. Department of Agriculture Animal and Plant Health Inspection Service Wildlife Services P.O. Box 786 Volcano, Hawaii 96785 808 985-7275 808 985-7276 (FAX) email tohashi(@,lava.net Eleutherodactylus Frog Eradication Plan 8 VI Bibliography Campbell, E.W. 2001 a. Dermal toxicity of selected agricultural pesticides, pharmaceutical products, and household chemicals to introduce Eleutherodactylus frogs in Hawaii - Draft NWRC, USDA-APHIS-WS, Fort Collins, CO. Campbell, E.W. 2001 b. Field efficacy trials of the directed spray application of caffeine solutions for controlling introduced Eleutherodactylus frogs in flouriculture and nursery crops in Hawaii - Draft. NWRC, USDA- APHIS-WS, Fort Collins, CO. Hawaii Agriculture Statistics Service (HA SS). 2000. Statistics of Hawaii Agriculture 1999. Honolulu HI Hawaii Export Nursery Association. 2001. Proposed Plan for Controlling the Spread of Caribbean Tree Frogs. Hawaii Department of Agriculture. 2001. Pesticide Trials for Coqui Frogs. August 20, 2001, Plant Industry Division, Honolulu HI Hawaii Department of Agriculture and Hawaii Department of Land and Natural Resources. 2001. Request for Specific Exemption Under Section 18 of FIFRA to Allow Field Use of Caffeine to Control the Coqui and Greenhouse Frogs in Floriculture and Nursery Crops, Residential Areas, Parks, Hotels, Resorts and W"w Forest Habitats in the State of Hawaii. Eleutherodactylus Frog Eradication Plan 9 Kraus, F., E.W. Campbell, A. Allison, and T. Pratt. 1999. Eleutherodactylus frog introductions in Hawaii. Herp. Rev. 30:211-25. Eleutherodactylus Frog Eradication Plan 10